Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Mao-to Prolongs the Survival of and Reduces TNF-α Expression in Mice with Viral Myocarditis

doi: 10.1093/ecam/nen010

Figure Lengend Snippet: Myofiber diameter, cardiac histopathologic scores and grading of cardiac expression of TNF-α protein in murine viral myocarditis. Groups as are described in Fig. 2. Grades of severity of necrosis and mononuclear cell infiltration on a scale from 1 to 4 are as follows: grade 1, lesions involving <25% of the ventricular myocardium; grade 2, lesions involving 25-50% of the myocardium; grade 3, lesions involving 50-75% of the myocardium; and grade 4, lesions involving 75-100% of the myocardium. ND; not determined, * P < 0.05 vs. group N, Δ P < 0.05 vs. group A.

Article Snippet: Next, the slides were incubated with an antibody directed against murine TNF-α (Alpha-Diagnostic International Inc., San Antonio, USA).

Techniques: Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Mao-to Prolongs the Survival of and Reduces TNF-α Expression in Mice with Viral Myocarditis

doi: 10.1093/ecam/nen010

Figure Lengend Snippet: A . Pathologic findings of the heart in mice after viral inoculation. Myocardial necrosis and immune cell infiltration was observed on day 6. However, myocardial necrosis in group C on day 6 was reduced compared with the groups A, B and D. B . Immunohistochemical findings showed cardiac expression of TNF-α in the heart. Myofibers in the group C were less positive than in the groups A, B or D. Group A: administered with 0.1 ml saline starting on day 0 to day 6, group B: administered with 0.1 ml Mao-to starting on day 0 to day 4, group C: administered with 0.1 ml Mao-to starting on day 2 to day 6, group D: administered 0.1 ml Mao-to from day 5 to day 10.

Article Snippet: Next, the slides were incubated with an antibody directed against murine TNF-α (Alpha-Diagnostic International Inc., San Antonio, USA).

Techniques: Immunohistochemical staining, Expressing, Saline

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Mao-to Prolongs the Survival of and Reduces TNF-α Expression in Mice with Viral Myocarditis

doi: 10.1093/ecam/nen010

Figure Lengend Snippet: Comparative expression of cardiac TNF-α mRNA. This expression in the group C were significantly less than in the groups A, B or D. Group A: administered with 0.1 ml saline starting on day 0 to day 6, group B: administered with 0.1 ml Mao-to starting on day 0 to day 4, group C: administered with 0.1 ml Mao-to starting on day 2 to day 6, group D: administered 0.1 ml Mao-to from day 5 to day 10.

Article Snippet: Next, the slides were incubated with an antibody directed against murine TNF-α (Alpha-Diagnostic International Inc., San Antonio, USA).

Techniques: Expressing, Saline

Journal:

Article Title: Late-Onset Chronic Inflammatory Encephalopathy in Immune-Competent and Severe Combined Immune-Deficient (SCID) Mice with Astrocyte-Targeted Expression of Tumor Necrosis Factor

doi:

Figure Lengend Snippet: Analysis of TNF-α expression. A: Transgene versus endogenous TNF-α expression was distinguished using target-specific probes generated as described in Materials and Methods. Organs were removed from control mice (C) or presymptomatic (P) and symptomatic (S) GT-2 mice. Total RNA (10 μg) was then used for analysis by RPA. B: In situ hybridization and immunohistochemistry was performed as described in Materials and Methods. In situ hybridization was used to detect TNF-α and combined with immunohistochemical staining for GFAP. Co-localization of TNF-α RNA and GFAP protein is shown for astrocytes (arrows) in the cerebellum of a GT-8 transgenic mouse. Original magnification, ×500. C: Immunohistochemical stain for TNF-α demonstrates expression of this protein in cells (arrow) with astrocyte morphology in the cerebellum of a GT-8 transgenic mouse. Original magnification, ×500.

Article Snippet: After blocking, slides were incubated overnight at room temperature with a polyclonal rabbit anti-murine TNF-α antibody (Genzyme, Cambridge, MA) diluted 1:1000 in the blocking buffer.

Techniques: Expressing, Generated, In Situ Hybridization, Immunohistochemistry, Immunohistochemical staining, Staining, Transgenic Assay